Truncated N-glycans affect protein folding in the ER of CHO-derived mutant cell lines without preventing calnexin binding
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Truncated N-glycans affect protein folding in the ER of CHO-derived mutant cell lines without preventing calnexin binding.
The involvement of N-glycans in the folding of influenza virus hemagglutinin (HA) was analyzed in two CHO-derived glycosylation mutants exhibiting a thermosensitive defect for secretion of human placental alkaline phosphatase. Truncated Man(5)GlcNAc(2)oligosaccharides with one or three glucose residues are attached to proteins of the MadIA214 and B3F7AP2-1 mutant cells, respectively. Newly synt...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 2000
ISSN: 1460-2423
DOI: 10.1093/glycob/10.1.77